Issue 32, 2023

A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

Abstract

We have developed a straightforward, one-pot, low-temperature hydrothermal method to transform oyster shell waste particles (bCCP) from the species Crassostrea gigas (Mg-calcite, 5 wt% Mg) into hydroxyapatite (HA) micro/nanoparticles. The influence of the P reagents (H3PO4, KH2PO4, and K2HPO4), P/bCCP molar ratios (0.24, 0.6, and 0.96), digestion temperatures (25–200 °C), and digestion times (1 week–2 months) on the transformation process was thoroughly investigated. At 1 week, the minimum temperature to yield the full transformation significantly reduced from 160 °C to 120 °C when using K2HPO4 instead of KH2PO4 at a P/bCCP ratio of 0.6, and even to 80 °C at a P/bCCP ratio of 0.96. The transformation took place via a dissolution–reprecipitation mechanism driven by the favorable balance between HA precipitation and bCCP dissolution, due to the lower solubility product of HA than that of calcite at any of the tested temperatures. Both the bCCP and the derived HA particles were cytocompatible for MG-63 human osteosarcoma cells and m17.ASC murine mesenchymal stem cells, and additionally, they promoted the osteogenic differentiation of m17.ASC, especially the HA particles. Because of their physicochemical features and biological compatibility, both particles could be useful osteoinductive platforms for translational applications in bone tissue engineering.

Graphical abstract: A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

  • This article is part of the themed collection: #MyFirstJMCB

Supplementary files

Article information

Article type
Paper
Submitted
17 Apr 2023
Accepted
07 Jul 2023
First published
21 Jul 2023
This article is Open Access
Creative Commons BY-NC license

J. Mater. Chem. B, 2023,11, 7766-7777

A sustainable one-pot method to transform seashell waste calcium carbonate to osteoinductive hydroxyapatite micro-nanoparticles

R. Fernández-Penas, C. Verdugo-Escamilla, C. Triunfo, S. Gärtner, A. D'Urso, F. Oltolina, A. Follenzi, G. Maoloni, H. Cölfen, G. Falini and J. Gómez-Morales, J. Mater. Chem. B, 2023, 11, 7766 DOI: 10.1039/D3TB00856H

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