Issue 24, 2023

Protein cohabitation: long-term immunoglobulin G storage at room temperature

Abstract

Long-term functional storage of therapeutic proteins at room temperature has been an eternal challenge. Inspired by the cellular cooperativity of proteins, we have taken a step forward to address this challenge by cohabitating Immunoglobulin G (IgG1) with a food protein gelatin in the solid-state at room temperature. Interestingly, IgG1 remained functionally active for a record 14 months revealed from the western-blot assay. Further quantification by HP-LC analysis showed 100% structural integrity of IgG1 with no degradation in the gelatin matrix during this period. The developed formulation has a direct application in oral medical nutrition therapy to cure gastrointestinal microbial infections. Also the strategy provides a robust energy economic alternative to the protein engineering methods for long-term functional storage of therapeutic proteins at room temperature.

Graphical abstract: Protein cohabitation: long-term immunoglobulin G storage at room temperature

Supplementary files

Article information

Article type
Communication
Submitted
27 Jan 2023
Accepted
15 May 2023
First published
23 May 2023
This article is Open Access
Creative Commons BY license

J. Mater. Chem. B, 2023,11, 5400-5405

Protein cohabitation: long-term immunoglobulin G storage at room temperature

P. Bharmoria, S. A. Ooi, A. Cellini, D. Tietze, M. Maj, K. Moth-Poulsen and A. A. Tietze, J. Mater. Chem. B, 2023, 11, 5400 DOI: 10.1039/D3TB00161J

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