Dye-surfactant co-assembly as the chromogenic indicator for nanomolar level detection of Cu(i) ions via a color-changing response

Abstract

Polyaromatic amphiphilic probes have been developed, that can be involved in chromogenic detection of Cu⁺ ions in anionic micelles. A rapid change in solution color from yellow to orange was observed in the presence of Cu⁺ ions. The detection limit was found at the nanomolar range. To the best of our knowledge, this is the first report of the visible detection of Cu⁺ ions in aqueous medium using anionic micelles as a stabilizing agent. Interestingly, the compound can also detect Cu⁺ ions, generated in situ from physiological redox processes. The mechanistic investigation suggests that the probe molecule forms a diamagnetic tetrahedral complex with the Cu⁺ ion, coordinating through a pyridyl ketone unit. In addition, we have also followed the interaction with Cu⁺ on a bilayer surface made of anionic phospholipids. Further, a Cu²⁺-probe ensemble is used to assay the reducing ability of different biogenic thiols depending upon the pK_a of their sulfhydryl (–SH) group. This allows us to determine the amount of reducing thiols present in human urine samples. Considering the high sensitivity of the present system, we screened water samples collected from different natural sources for Cu⁺ ions. Nearly 100% recovery values with considerably small relative standard deviations (<5%) indicate that the present system is indeed suitable for real-life sample analysis. Finally, low-cost, reusable, chemically-modified paper strips have been developed for rapid, on-location detection of Cu⁺ ions.