Issue 25, 2023

Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

Abstract

Multiplex technology is an important emerging field, in diagnostic sciences, that enables the simultaneous detection of several analytes in a single sample. The light-emission spectrum of a chemiluminescent phenoxy-dioxetane luminophore can be accurately predicted by determining the fluorescence-emission spectrum of its corresponding benzoate species, which is generated during the chemiexcitation process. Based on this observation, we designed a library of chemiluminescent dioxetane luminophores with multicolor emission wavelengths. Two dioxetane luminophores that have different emission spectra, but similar quantum yield properties, were selected from the synthesized library for a duplex analysis. The selected dioxetane luminophores were equipped with two different enzymatic substrates to generate turn-ON chemiluminescent probes. This pair of probes exhibited a promising ability to act as a chemiluminescent duplex system for the simultaneous detection of two different enzymatic activities in a physiological solution. In addition, the pair of probes were also able to simultaneously detect the activities of the two enzymes in a bacterial assay, using a blue filter slit for one enzyme and a red filter slit for the other enzyme. As far as we know, this is the first successful demonstration of a chemiluminescent duplex system composed of two-color phenoxy-1,2-dioxetane luminophores. We believe that the library of dioxetanes presented here will be beneficial for developing chemiluminescence luminophores for multiplex analysis of enzymes and bioanalytes.

Graphical abstract: Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

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Article information

Article type
Edge Article
Submitted
10 May 2023
Accepted
26 May 2023
First published
01 Jun 2023
This article is Open Access

All publication charges for this article have been paid for by the Royal Society of Chemistry
Creative Commons BY-NC license

Chem. Sci., 2023,14, 6953-6962

Chemiluminescent duplex analysis using phenoxy-1,2-dioxetane luminophores with color modulation

S. Gutkin, R. Tannous, Q. Jaber, M. Fridman and D. Shabat, Chem. Sci., 2023, 14, 6953 DOI: 10.1039/D3SC02386A

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