The first spectrofluorimetric protocol for sensitive quantitative analysis of bromocriptine in its pure and pharmaceutical forms: evaluation of the greenness of the method

Abstract

Bromocriptine mesylate, a dopamine D₂ receptor agonist, has been quantitatively determined using a sensitive, precise, quick, and affordable spectrofluorimetric method. The proposed method relies on the estimation of bromocriptine native fluorescence after the optimization of different factors to improve its inherently weak fluorescence through the use of a sodium dodecyl sulphate micellar system (2% w/v). Following excitation at 238 nm, the enhanced fluorescence intensity of bromocriptine was determined at 418 nm. As compared to its native fluorescence, the bromocriptine fluorescence intensity has been greatly enhanced by about 15 fold by employing the micellar system. The plot of intensity of fluorescence versus bromocriptine concentration was linear in the range of 50–600 ng mL⁻¹. The method was found to have a high sensitivity, as indicated by the low limit of detection and limit of quantitation values (14.57 and 44.16 ng mL⁻¹ respectively). Without the interference of any excipient, this method was effectively employed to quantify bromocriptine in its pharmaceutical dosage form.