Issue 46, 2023, Issue in Progress

Development of a novel hypochlorite ratio probe based on coumarin and its application in living cells

Abstract

Hypochlorous acid is a reactive oxygen species that is widely present in the body and has been found to exhibit an elevated concentration in tumors. As a result, fluorescent probes for tumor detection have recently gained significant attention. In this study, we designed and synthesized a novel ratiometric fluorescent probe, LW-1, using coumarin as a scaffold, and characterized its spectral properties. LW-1 displayed indigo blue fluorescence at low concentrations of hypochlorous acid. As the concentration of hypochlorous acid increased, the probe underwent a reaction, resulting in a red shift in its fluorescence peak and exhibiting green fluorescence. The fluorescence intensity ratio (green/blue) was a susceptible detection signal for HClO. LW-1 exhibited favorable characteristics, including a low detection limit, high sensitivity, good stability, and low background interference. The detection limit has reached 2.4642 nM. Moreover, we successfully employed LW-1 to image normal human liver and colon cancer cells in vitro, demonstrating its potential as a promising tool for tumor detection. Overall, our findings suggest that LW-1 could serve as a valuable addition to the current arsenal of fluorescent probes for tumor detection, with potential applications in the diagnosis and treatment of cancer.

Graphical abstract: Development of a novel hypochlorite ratio probe based on coumarin and its application in living cells

Supplementary files

Article information

Article type
Paper
Submitted
14 Jul 2023
Accepted
17 Oct 2023
First published
03 Nov 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 32518-32522

Development of a novel hypochlorite ratio probe based on coumarin and its application in living cells

H. Wen, Z. Liu, Z. Su, Jamal. A. H. Kowah, E. Hao and X. Liu, RSC Adv., 2023, 13, 32518 DOI: 10.1039/D3RA04729F

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