Enhanced volatile fatty acid production from waste activated sludge by urea hydrogen peroxide: performance and mechanisms

Abstract

Anaerobic acidogenesis of waste activated sludge (WAS) presents significant potential for resource recovery and waste treatment. However, the slow hydrolysis of WAS limits the efficiency of this approach. In this study, we applied urea hydrogen peroxide (UHP) pretreatment to enhance WAS hydrolysis and investigated the effects of operating parameters on volatile fatty acid (VFA) production and the associated mechanisms. Results demonstrated that UHP significantly improved WAS hydrolysis and VFA production, with a three-fold increase in soluble chemical oxygen demand (SCOD) compared to the control group. UHP dosage emerged as the most critical factor for VFA production, with the maximum VFA concentration increasing from 1127.6 to 8800.9 mg COD per L as UHP dosage ranged from 0 to 6 mmol g⁻¹ VSS (Volatile suspended solids). At an optimal UHP dosage of 4 mmol g⁻¹ VSS, both the unit oxidant promotion efficiency (ΔVFAs/ΔUHP) and the maximum VFA concentration reached relatively high levels, at 35.3 mg COD per mmol and 7527.3 mg COD per L, respectively. UHP pretreatment generated alkaline conditions, H₂O₂, ·OH and free ammonia, which collectively disrupted the extracellular polymeric substances (EPS) structure, transforming unextractable EPS into extractable forms and promoting the release of organic matter during both the pretreatment and fermentation stages. Excitation-emission matrix (EEM) analysis revealed that UHP increased the concentration of easily utilizable organic matter, providing more substrates for acidogenic bacteria and enhancing VFA production. Furthermore, weak alkaline conditions and high free ammonia concentrations in the UHP group facilitated VFA accumulation by preventing rapid acidification and suppressing methanogen activity. This study offers valuable insights into the potential of UHP pretreatment for enhancing WAS hydrolysis and VFA production, with promising applications in wastewater treatment and resource recovery.