Nitrogen-doped carbon dots enhanced seedling growth and salt tolerance with distinct requirements of excitation light

Abstract

Numerous nanomaterials with optical properties have demonstrated excellent capacities to enhance plant growth and stress tolerance. However, the corresponding mechanisms have only been partially characterized, especially the excitation-light dependencies of different actions. Here, nitrogen-doped carbon dots (N-CDs) were developed to explore the excitation-light dependence in N-CD-induced growth enhancement and salt tolerance. Compared to the control, N-CDs induced significant enhancements in Arabidopsis thaliana growth under excitation light, including fresh/dry weight of shoot (21.07% and 16.87%), chlorophyll content (9.17%), soluble sugar content (23.41%), leaf area (28.68%), total root length (34.07%) and root tip number (46.69%). In the absence of excitation light, N-CD-treated seedlings exhibited little differences in these parameters, except the enhancements in root length (24.51%) and root tip number (10.24%). On the other hand, N-CD-treatment could improve seedling salt tolerance with or without excitation light. Under salt stress (150 mM NaCl), in the presence of excitation light, the N-CDs treatment significantly increased shoot/root fresh weight and chlorophyll content by 43.29%, 50.66% and 22.59%, and reduced malondialdehyde (MDA) content and relative conductivity by 17.59% and 32.58% compared to the control group. In the absence of excitation light, significant enhancements in shoot/root fresh weight (34.22%, 32.60%) and chlorophyll content (10.45%), and obvious decreases in MDA content (28.84%) and relative conductivity (16.13%) were also found. These results indicated that N-CDs only induced growth enhancement under excitation light, but they improved salt tolerance with and without excitation light, suggesting that the two effects occurred via distinct signaling pathways. This study revealed the excitation-light dependencies of nanomaterial-involved agriculture applications, providing insight into designing more efficient nanomaterials in the future.