Issue 29, 2023

Development of an activity-based chemiluminogenic probe for γ-glutamylcyclotransferase

Abstract

While γ-glutamylcyclotransferase (GGCT) has been implicated in cancer-cell proliferation, the role of GGCT enzymatic activity in the regulation of cancer-cell growth remains unclear. Toward further understanding of GGCT in vivo, here we report a novel cell-permeable chemiluminogenic probe “MAM-LISA-103” that detects intracellular GGCT activity and apply it to in vivo imaging. We first developed a chemiluminogenic probe LISA-103, which simply and sensitively detects the enzymatic activity of recombinant GGCT through chemiluminescence. We then designed the cell-permeable GGCT probe MAM-LISA-103 and applied it to several biological experiments. MAM-LISA-103 successfully detected the intracellular GGCT activity in GGCT-overexpressing NIH-3T3 cells. Moreover, MAM-LISA-103 demonstrated tumor-imaging ability when administered to a xenograft model using immunocompromised mice inoculated with MCF7 cells.

Graphical abstract: Development of an activity-based chemiluminogenic probe for γ-glutamylcyclotransferase

Supplementary files

Article information

Article type
Paper
Submitted
27 Apr 2023
Accepted
21 Jun 2023
First published
05 Jul 2023

Org. Biomol. Chem., 2023,21, 5977-5984

Development of an activity-based chemiluminogenic probe for γ-glutamylcyclotransferase

Y. Nohara, K. Taniguchi, H. Ii, S. Masuda, H. Kawakami, M. Matsumoto, Y. Hattori, S. Kageyama, T. Sakai, S. Nakata and T. Yoshiya, Org. Biomol. Chem., 2023, 21, 5977 DOI: 10.1039/D3OB00655G

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