Resolving the complexity in human milk oligosaccharides using pure shift NMR methods and CASPER†
Marshall J.
Smith, 
a
Emma L.
Gates,
a
Göran
Widmalm,
b
Ralph W.
Adams,
a
Gareth A.
Morris
a
and
Mathias
Nilsson
*a
a
Emma L.
Gates,
a
Göran
Widmalm,
b
Ralph W.
Adams,
a
Gareth A.
Morris
a
and
Mathias
Nilsson
*a
Abstract
Human milk oligosaccharides belong to an important class of bioactive molecules with diverse effects on the development of infants. NMR is capable of providing vital structural information about oligosaccharides which can aid in determining structure–function relationships. However, this information is often concealed by signal overlap in 1H spectra, due to the narrow chemical shift range and signal multiplicity. Signal overlap in oligosaccharide spectra can be greatly reduced, and resolution improved, by utilising pure shift methods. Here the benefits of combining pure shift methods with the CASPER computational approach to resonance assignment in oligosaccharides are demonstrated.
- This article is part of the themed collections: Celebrating the 200th Anniversary of the University of Manchester and Celebrating the scientific accomplishments of RSC Fellows
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