Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)3/(KIAALKE)3 and (EIAALEK)3/(RIAALRE)3 coiled-coil systems

D. Aerssens; L. Miret-Casals; D. Gomez; D. Sousa-Neves; Y. Levy; M. De Vleesschouwer; A. Manicardi; A. Madder

doi:10.1039/D2OB02181A

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Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems†

D. Aerssens,‡^a L. Miret-Casals,

‡*^a D. Gomez,^b D. Sousa-Neves,^a Y. Levy,

^c M. De Vleesschouwer,^a A. Manicardi

*^ad and A. Madder

*^a

Author affiliations

* Corresponding authors

^a Organic and Biomimetic Chemistry Research Group, Department of Organic and Macromolecular Chemistry, Ghent University, Krijgslaan 281-S4, Gent 9000, Belgium
E-mail: Annemieke.madder@ugent.be, Alex.Manicardi@unipr.it, Laia.MiretCasals@ugent.be

^b Department of Biology and Chemistry, New York University, New York 10003, USA

^c Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel

^d Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Viale delle Scienze, 17/A, I-43124 Parma, Italy

Abstract

We describe for the first time the co-existence of the parallel and antiparallel conformation of the heterodimeric E3/K3 and E3/R3 coiled-coil systems in solution. The introduction of a furanylated amino acid in the (EIAALEK)₃ sequence allowed, upon photo-induced covalent crosslinking, freezing of the respective coiled-coil complexes present in solution. The occurrence of both parallel and antiparallel conformations in solution was supported by computational simulations and further confirmed by fluorescence experiments based on pyrene–pyrene stacking.

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Article information

DOI: https://doi.org/10.1039/D2OB02181A
Article type: Paper
Submitted: 30 Nov 2022
Accepted: 20 Jun 2023
First published: 21 Jun 2023

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Org. Biomol. Chem., 2023,21, 5648-5657

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Photo-induced crosslinking uncovers an antiparallel strand orientation in heterodimeric (EIAALEK)₃/(KIAALKE)₃ and (EIAALEK)₃/(RIAALRE)₃ coiled-coil systems

D. Aerssens, L. Miret-Casals, D. Gomez, D. Sousa-Neves, Y. Levy, M. De Vleesschouwer, A. Manicardi and A. Madder, Org. Biomol. Chem., 2023, 21, 5648 DOI: 10.1039/D2OB02181A

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Organic & Biomolecular Chemistry