Issue 48, 2023

Self-decorating cells via surface-initiated enzymatic controlled radical polymerization

Abstract

Through the innovative use of surface-displayed horseradish peroxidase, this work explores the enzymatic catalysis of both bioRAFT polymerization and bioATRP to prompt polymer synthesis on the surface of Saccharomyces cerevisiae cells, with bioATRP outperforming bioRAFT polymerization. The resulting surface modification of living yeast cells with synthetic polymers allows for a significant change in yeast phenotype, including growth profile, aggregation characteristics, and conjugation of non-native enzymes to the clickable polymers on the cell surface, opening new avenues in bioorthogonal cell-surface engineering.

Graphical abstract: Self-decorating cells via surface-initiated enzymatic controlled radical polymerization

Supplementary files

Article information

Article type
Communication
Submitted
10 Aug 2023
Accepted
30 Nov 2023
First published
01 Dec 2023
This article is Open Access
Creative Commons BY license

Nanoscale, 2023,15, 19486-19492

Self-decorating cells via surface-initiated enzymatic controlled radical polymerization

A. Belluati, D. Happel, M. Erbe, N. Kirchner, A. Szelwicka, A. Bloch, V. Berner, A. Christmann, B. Hertel, R. Pardehkhorram, A. Reyhani, H. Kolmar and N. Bruns, Nanoscale, 2023, 15, 19486 DOI: 10.1039/D3NR04008A

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