Issue 22, 2023

An ultrasensitive electrochemiluminescence immunosensor for SARS-CoV-2 nucleocapsid protein detection based on signal amplification strategy of DMSN@QDs

Abstract

Based on signal amplification strategy of dendritic mesoporous silica nanospheres loaded with CdSe/ZnS quantum dots (DMSN@QDs), an ultrasensitive electrochemiluminescence (ECL) immunosensor with magnetic separation was constructed for the detection of SARS-CoV-2 nucleocapsid protein (NP). DMSN, a mesoporous material with abundant radial pores, large specific surface area and high porosity, can increase the loading capacity of QDs and hinder their aggregation as the nanocarrier. DMSN@QDs with good ECL efficiency were used as signal labels to construct a sandwich immunosensor. The designed ECL immunosensor displayed a good linear relationship for NP concentrations ranging from 0.005 ng mL−1 to 50 ng mL−1, with a limit of detection of 3.33 pg mL−1. The ECL immunosensor was successfully applied to detect NP in human serum samples with satisfactory recovery. This strategy provided a new method for detecting NP and expanded the application field of DMSN.

Graphical abstract: An ultrasensitive electrochemiluminescence immunosensor for SARS-CoV-2 nucleocapsid protein detection based on signal amplification strategy of DMSN@QDs

Supplementary files

Article information

Article type
Paper
Submitted
08 Apr 2023
Accepted
12 May 2023
First published
22 May 2023

New J. Chem., 2023,47, 10706-10714

An ultrasensitive electrochemiluminescence immunosensor for SARS-CoV-2 nucleocapsid protein detection based on signal amplification strategy of DMSN@QDs

S. Feng, F. Pei, W. Hu, Z. Liu, B. Du, X. Mu, B. Liu, Q. Hao, W. Lei and Z. Tong, New J. Chem., 2023, 47, 10706 DOI: 10.1039/D3NJ01641B

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