Isolation of cytotoxic daphnane-type and tigliane-type diterpenoids from Daphne genkwa using MetGem software

Abstract

To discover diterpenoids that naturally possess chemical weapons against cancer, the biological and chemical diversity of the flower buds of Daphne genkwa Sieb. et Zucc was investigated. Guided by LC-MS/MS analysis and the MetGem Molecular Networking, eight daphnane-type and tigliane-type diterpenoids including four new compounds were isolated. Spectroscopic elucidation and ECD quantum chemical calculations revealed their structures. All compounds were evaluated their cytotoxic activity of A549, Hep3B, and MCF-7 cell lines. Compounds 1 and 8 showed potent activity against the A549 and Hep3B cells.