Issue 19, 2023

Chitosan: a green adhesive for surface functionalization and fabrication of thermoplastic biomedical microdevices

Abstract

Chitosan (CS) is a natural polymer that exhibits many biological properties and is used as a biomaterial for antibacterial coatings, tissue engineering, cell research, drug delivery, and negatively charged molecule capture. In our previous study, we used a CS–polydopamine mixture to realize UV-assisted bonding between poly(methyl methacrylate) (PMMA) substrates to fabricate microdevices for self-assembled stem cell spheroid cultures. Herein, we attained reliable adhesive bonding between PMMAs using CS at room temperature assisted by oxygen plasma. The bond strength of adhesion was as high as 2.1 MPa, which could be stable for over two months according to the leak test. The adhesive bonding and surface functionalization of the microchannels were simultaneously completed such that the microdevices could be directly used for mesenchymal stem cell culture for spheroid generation and DNA purification for point-of-care testing (POCT) devices. Surface characterization was performed by contact angle measurements, Fourier-transform infrared spectroscopy, scanning electron microscopy, and atomic force microscopy. The POCT device allows sequential on-chip DNA purification, amplification, and colorimetric detection of pathogenic bacteria. This method provides a convenient and reliable strategy for the fabrication of PMMA microdevices that can be directly implemented in biological studies and POCT applications without involving prior surface modification steps.

Graphical abstract: Chitosan: a green adhesive for surface functionalization and fabrication of thermoplastic biomedical microdevices

Supplementary files

Article information

Article type
Paper
Submitted
08 Jun 2023
Accepted
11 Aug 2023
First published
11 Aug 2023

Lab Chip, 2023,23, 4245-4254

Chitosan: a green adhesive for surface functionalization and fabrication of thermoplastic biomedical microdevices

K. T. L. Trinh, D. A. Thai, D. H. Yang and N. Y. Lee, Lab Chip, 2023, 23, 4245 DOI: 10.1039/D3LC00500C

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