Issue 13, 2023

In situ investigation of detoxification and metabolic effects of polyfluoroalkyl substances on metal–organic frameworks combined with cell-cultured microfluidics

Abstract

Over 9000 types of per- and polyfluoroalkyl substances (PFASs) have been produced that exhibit environmental persistence, bioaccumulation and biotoxicity, and pose a potential hazard to human health. Although metal–organic frameworks (MOFs) are promising structure-based materials for adsorbing PFASs, the enormous structural diversity and variability of the pharmacologic action of PFASs present challenges to the development of structure-based adsorbents. To address this issue, we propose an in situ platform for the high-throughput identification of efficient MOF sorbents that can adsorb PFASs and their metabolism using a filter-chip-solid phase extraction-mass spectrometry (SPE-MS) system. As a proof of concept, we screened BUT-16 as an attractive material for in situ fluorotelomer alcohol (FTOH) adsorption. The results demonstrated that FTOH molecules were adsorbed around the surface of the large hexagonal pores of BUT-16 by forming multiple hydrogen bonding interactions with its Zr6 clusters. The FTOH removal efficiency of the BUT16 filter was 100% over a period of 1 min. To determine the FTOH metabolism effects in different organs, HepG2 human hepatoma, HCT116 colon cancer, renal tubular HKC, and vascular endothelial HUVEC cells were cultured on a microfluidic chip, and SPE-MS was used to track a variety of cell metabolites in real time. Overall, the filter-Chip-SPE-MS system is a versatile and robust platform for the real-time monitoring of noxious pollutant detoxification, biotransformation, and metabolism, which facilitates pollutant antidote development and toxicology assay.

Graphical abstract: In situ investigation of detoxification and metabolic effects of polyfluoroalkyl substances on metal–organic frameworks combined with cell-cultured microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
15 May 2023
Accepted
24 May 2023
First published
24 May 2023
This article is Open Access
Creative Commons BY license

Lab Chip, 2023,23, 3062-3069

In situ investigation of detoxification and metabolic effects of polyfluoroalkyl substances on metal–organic frameworks combined with cell-cultured microfluidics

N. Xu, H. Lin, Q. Du, S. Dong, J. Cheng, P. Wang and J. Lin, Lab Chip, 2023, 23, 3062 DOI: 10.1039/D3LC00423F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements