Issue 15, 2023

Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

Abstract

Viral load quantitation is useful in clinical point-of-care settings to assess the status of patients with infectious disease, track response to treatment, and estimate infectiousness. However, existing methods for quantitating viral loads are complex and difficult to integrate into these settings. Here, we describe a simple, instrument-free approach for viral load quantitation suitable for point-of-care use. We develop a shaken digital droplet assay that can quantitate SARS-CoV2 with sensitivity comparable to gold standard qPCR.

Graphical abstract: Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

Article information

Article type
Paper
Submitted
02 Feb 2023
Accepted
20 Apr 2023
First published
11 Jul 2023
This article is Open Access
Creative Commons BY-NC license

Lab Chip, 2023,23, 3479-3486

Viral load quantitation at the point-of-care with shaken digital droplet RT-LAMP

D. W. Weisgerber, K. Langer, V. Servellita, P. Xu, C. Y. Chiu and A. R. Abate, Lab Chip, 2023, 23, 3479 DOI: 10.1039/D3LC00096F

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