Issue 6, 2023

Cascading and precise regulation of the selective bio-production of 2- or 5-ketogluconic acid from glucose with whole-cell catalysis technology

Abstract

Ketogluconic acid is widely applied in several industries as the bio-precursor of vitamin C and tartaric acid, while the selective production of 2-ketogluconic acid (2-kGA) or 5-ketogluconic acid (5-kGA) from glucose is so far still a challenge for chemical and biotechnical processes. The latest studies have demonstrated that Gluconobacter oxydans effectively catalyzes glucose to a mixture of gluconic acid, 2-kGA and 5-kGA under moderate conditions. In order to develop a novel and efficient technology for the selective production of 2-kGA or 5-kGA without by-products, cascading and precise regulation with calcium cations was explored and investigated using whole-cell catalysis by G. oxydans. CaCO3 was used as a neutralizer, which not only intensified the whole-cell catalysis to a better productivity, but also significantly reduced the solubility of 5-kGA. The in situ separation of 5-kGA-Ca was achieved with a crystallization yield of 97.3% thanks to the different solubilities of kGA-Ca salts. The 2-kGA-Ca crystals were subsequently obtained with a crystallization yield of 94.3% from the bacterial fermentation broth. The high purity of the two kGA salt crystals was confirmed by chromatography and NMR, respectively. Finally, totals of 640.2 g of 2-kGA and 249.2 g of 5-kGA were obtained by the addition of 1000 g of glucose in the processing experiment. This cascading and precise regulation method might provide a promising technical approach for the selective bio-production of 2- or 5-kGA and glucose bioconversion.

Graphical abstract: Cascading and precise regulation of the selective bio-production of 2- or 5-ketogluconic acid from glucose with whole-cell catalysis technology

Supplementary files

Article information

Article type
Paper
Submitted
01 Dec 2022
Accepted
13 Feb 2023
First published
14 Feb 2023

Green Chem., 2023,25, 2378-2386

Cascading and precise regulation of the selective bio-production of 2- or 5-ketogluconic acid from glucose with whole-cell catalysis technology

X. Hua, J. Han, X. Liu and Y. Xu, Green Chem., 2023, 25, 2378 DOI: 10.1039/D2GC04566D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements