Issue 27, 2023

GFP-related chromophores: photoisomerization, thermal reversion, and DNA labelling

Abstract

Due to the pronounced effect of the confined environment on the photochemical properties of 4-hydroxybenzylidene imidazolinone (HBI), a GFP-related chromophore, imidazolidinone and imidazothiazolone analogues have been studied as fluorescent probes. Their photoisomerization and their thermal reversion were studied under 365-nm-irradiation, resulting in observation of an enthalpy–entropy compensation effect. Theoretical studies were carried out to shed light on the thermal reversion mechanism. Moreover, photophysical studies of benzylidene imidazothiazolone in the presence of dsDNA revealed fluorescence enhancement. The prepared compounds could be considered as a valuable tool for the detailed investigation of physicochemical, biochemical, or biological systems.

Graphical abstract: GFP-related chromophores: photoisomerization, thermal reversion, and DNA labelling

Supplementary files

Article information

Article type
Paper
Submitted
11 Apr 2023
Accepted
08 Jun 2023
First published
09 Jun 2023

Phys. Chem. Chem. Phys., 2023,25, 17943-17951

Author version available

GFP-related chromophores: photoisomerization, thermal reversion, and DNA labelling

M. S. Faillace, E. A. Dolgopolova, N. M. Ceballos, E. N. Ruiz Pereyra, L. Lanfri, G. A. Argüello, M. Burgos Paci, N. B. Shustova and W. J. Peláez, Phys. Chem. Chem. Phys., 2023, 25, 17943 DOI: 10.1039/D3CP01655B

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