Issue 24, 2023

ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics

Abstract

An NMR NOAH-supersequence is presented consisting of five CEST experiments for studying protein backbone and side-chain dynamics by 15N-CEST, carbonyl-13CO-CEST, aromatic-13Car-CEST, 13Cα-CEST, and methyl-13Cmet-CEST. The new sequence acquires the data for these experiments in a fraction of the time required for the individual experiments, saving over four days of NMR time per sample.

Graphical abstract: ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics

Supplementary files

Article information

Article type
Communication
Submitted
06 Apr 2023
Accepted
10 May 2023
First published
08 Jun 2023
This article is Open Access
Creative Commons BY-NC license

Phys. Chem. Chem. Phys., 2023,25, 16217-16221

ARCHE-NOAH: NMR supersequence with five different CEST experiments for studying protein conformational dynamics

R. Cabrera Allpas, A. L. Hansen and R. Brüschweiler, Phys. Chem. Chem. Phys., 2023, 25, 16217 DOI: 10.1039/D3CP01580G

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