SPICY: a method for single scan rotating frame relaxometry

Abstract

T _1ρ is an NMR relaxation mode that is sensitive to low frequency molecular motions, making it an especially valuable tool in biomolecular research. Here, we introduce a new method, SPICY, for measuring T_1ρ relaxation times. In contrast to conventional T_1ρ experiments, in which the sequence is repeated many times to determine the T_1ρ time, the SPICY sequence allows determination of T_1ρ within a single scan, shortening the experiment time remarkably. We demonstrate the method using ¹H T_1ρ relaxation dispersion experiments. Additionally, we combine the sequence with spatial encoding to produce 1D images in a single scan. We show that T_1ρ relaxation times obtained using the single scan approach are in good agreement with those obtained using the traditional experiments.