Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

Shiying Zhou; Shuyu Zhu; Zhen Huang; Jian Chen; Jiawei Li; Mei Yang; Liang Jin; Danqun Huo; Changjun Hou

doi:10.1039/D3CC04223E

Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18†

Shiying Zhou,‡^a Shuyu Zhu,‡^a Zhen Huang,^b Jian Chen,^c Jiawei Li,

^c Mei Yang,

^a Liang Jin,*^a Danqun Huo*^a and Changjun Hou

*^ad

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* Corresponding authors

^a Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing 400044, PR China
Fax: +862365102507
Tel: +862365102507

^b Key Laboratory of Bio-Resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, SeNA Research Institute and Szostak-CDHT Large Nucleic Acids Institute, Chengdu, PR China

^c Chongqing University Three Gorges Hospital, Chongqing, PR China

^d Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineering, Chongqing University, Chongqing, PR China
E-mail: houcj@cqu.edu.cn

Abstract

Simultaneous detection of multiple targets can provide important data support for clinical diagnosis and treatment. Here, we report a facile isothermal assay based on target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a (TM-RCT/Cas12a-Cas13a). Through facile one-step amplification (TM-RCT), two target DNAs are converted to RNA amplified products. The simultaneous detection of HPV16 and HPV18 is then achieved by combining two CRISPR/Cas systems. This system shows excellent sensing performance and provides a universal method for simultaneous detection.

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DOI: https://doi.org/10.1039/D3CC04223E
Article type: Communication
Submitted: 29 Aug 2023
Accepted: 12 Sep 2023
First published: 13 Sep 2023

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Chem. Commun., 2023,59, 11987-11990

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Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

S. Zhou, S. Zhu, Z. Huang, J. Chen, J. Li, M. Yang, L. Jin, D. Huo and C. Hou, Chem. Commun., 2023, 59, 11987 DOI: 10.1039/D3CC04223E

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Chemical Communications

Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18†

Abstract

Supplementary files

Article information

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Target-mediated rolling circle transcription coupling with CRISPR/Cas12a-Cas13a for simultaneous detection of HPV16 and HPV18

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