EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(II)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.
Y. Shenberger, L. Gevorkyan-Airapetov, M. Hirsch, L. Hofmann and S. Ruthstein, Chem. Commun., 2023, 59, 10524 DOI: 10.1039/D3CC03047D
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