Semisynthetic maturation of [FeFe]-hydrogenase using [Fe2(μ-SH)2(CN)2(CO)4]2−: key roles for HydF and GTP†
Batuhan
Balci, 
a
Roark D.
O’Neill,
a
Eric M.
Shepard,
a
Adrien
Pagnier,
a
Alexander
Marlott,
a
Michael T.
Mock,
a
William E.
Broderick
a
and
Joan B.
Broderick
*a
a
Roark D.
O’Neill,
a
Eric M.
Shepard,
a
Adrien
Pagnier,
a
Alexander
Marlott,
a
Michael T.
Mock,
a
William E.
Broderick
a
and
Joan B.
Broderick
*a
Abstract
Here we describe maturation of the [FeFe]-hydrogenase from its [4Fe–4S]-bound precursor state by using the synthetic complex [Fe2(μ-SH)2(CN)2(CO)4]2− together with HydF and components of the glycine cleavage system, but in the absence of the maturases HydE and HydG. This semisynthetic and fully-defined maturation provides new insights into the nature of H-cluster biosynthesis.
![Graphical abstract: Semisynthetic maturation of [FeFe]-hydrogenase using [Fe2(μ-SH)2(CN)2(CO)4]2−: key roles for HydF and GTP](/en/Image/Get?imageInfo.ImageType=GA&imageInfo.ImageIdentifier.ManuscriptID=D3CC02169F&imageInfo.ImageIdentifier.Year=2023)
- This article is part of the themed collection: Chemical Communications HOT Articles 2023
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