Issue 45, 2023
From the journal:

Chemical Communications

Reusable electrochemiluminescence biosensor based on tetrahedral DNA signal amplification for ultrasensitive detection of microRNAs†

Jingjing Zhang,ab   Jingfeng Zhu,a   Fenglian Guo,b   Jinke Jiang,a   Mo Xie,b   Lin Hao*c  and  Jie Chao ORCID logo *ab  

* Corresponding authors

a Key Laboratory for Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts and Telecommunications, Nanjing 210023, China
E-mail: iamjchao@njupt.edu.cn

b School of Geographic and Biologic Information, Nanjing University of Posts and Telecommunications, Nanjing 210023, China

c Department of Urology, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu, China
E-mail: haolinxuzhou@163.com

Abstract

A novel and reusable electrochemiluminescence biosensor was developed based on tetrahedral DNA (TDN) signal amplification for ultrasensitive detection of miRNA-27a. The flowered nickel-iron layered double hydroxide@AuNPs (NiFe-LDH@AuNPs) composites increase the amount of hairpin DNA fixed on the electrode. When miRNA is present, TDN-Ru(bpy)32+ acts as an ECL probe, forming a stable sandwich structure with miRNA-27a and hairpin DNA through base complementation pairing, thus achieving miRNA detection. This biosensor has the characteristics of high sensitivity, excellent selectivity, and good reproducibility.

Graphical abstract: Reusable electrochemiluminescence biosensor based on tetrahedral DNA signal amplification for ultrasensitive detection of microRNAs

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Article information

DOI
https://doi.org/10.1039/D3CC01830J
Article type
Communication
Submitted
14 Apr 2023
Accepted
09 May 2023
First published
09 May 2023

Chem. Commun., 2023,59, 6869-6872

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Reusable electrochemiluminescence biosensor based on tetrahedral DNA signal amplification for ultrasensitive detection of microRNAs

J. Zhang, J. Zhu, F. Guo, J. Jiang, M. Xie, L. Hao and J. Chao, Chem. Commun., 2023, 59, 6869 DOI: 10.1039/D3CC01830J

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