Issue 60, 2023
From the journal:

Chemical Communications

Cell-type-specific CRISPRization of mitochondrial DNA using bifunctional biodegradable silica nanoparticles†

Linye Jiang,a   Bizhong Zhou,a   Huijuan Qian,a   Hongfeng Wang,a   Yuxi Wang,a   Weijiao Fan,b   Guowan Zheng*b  and  Jingyan Ge ORCID logo *a  

* Corresponding authors

a Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, P. R. China
E-mail: gejy@zjut.edu.cn

b Otolaryngology & Head and Neck Center, Cancer Center, Department of Head and Neck Surgery, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou 310014, P. R. China
E-mail: zhengguowan@hmc.edu.cn

Abstract

We report cell-type-specific and CRISPR/Cas9-mediated mtDNA editing platform by using bifunctional biodegradable silica nanoparticles, which were capable of selective intracellular delivery to CD44-overexpressed cells and subsequent mitochondrial localization, followed by glutathione-responsive biodegradation and release of Cas9/sgRNA to realize precise mtDNA editing.

Graphical abstract: Cell-type-specific CRISPRization of mitochondrial DNA using bifunctional biodegradable silica nanoparticles

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Article information

DOI
https://doi.org/10.1039/D3CC01778H
Article type
Communication
Submitted
11 Apr 2023
Accepted
26 Jun 2023
First published
27 Jun 2023

Chem. Commun., 2023,59, 9251-9254

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Cell-type-specific CRISPRization of mitochondrial DNA using bifunctional biodegradable silica nanoparticles

L. Jiang, B. Zhou, H. Qian, H. Wang, Y. Wang, W. Fan, G. Zheng and J. Ge, Chem. Commun., 2023, 59, 9251 DOI: 10.1039/D3CC01778H

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