Direct experimental evidence for the boronic acid-mediated bridging of DNA hybrids

Abstract

The use of terminal deoxynucleotidyl transferase for the first time in a mechanistic exploration—through colorimetric sensing and isothermal titration calorimetric studies—has provided direct experimental evidence of a boronic acid moiety bridging two DNA duplexes via the 3′ hydroxyl groups, offering new opportunities and insights into the domain of DNA (nano)biotechnology.