Issue 34, 2023

Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules

Abstract

We report that a selective fluorescent indicator NBD-NCD for UGGAA repeats resulted in fluorescence quenching upon binding to RNA and recovered the fluorescence by displacing NBD-NCD with UGGAA repeat-targeted small molecules. The fluorescent indicator displacement assay using NBD-NCD can detect the interaction of small molecules with UGGAA repeats.

Graphical abstract: Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules

Supplementary files

Article information

Article type
Communication
Submitted
22 Feb 2023
Accepted
29 Mar 2023
First published
31 Mar 2023
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2023,59, 5071-5074

Fluorescent indicator displacement assay for the discovery of UGGAA repeat-targeted small molecules

T. Shibata, Y. Matsumoto, A. Iihara, K. Yamada, H. Ochiai, R. Saito, S. Kusaka and T. Kume, Chem. Commun., 2023, 59, 5071 DOI: 10.1039/D3CC00837A

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