Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

Mrityunjay Gupta; Jingtian Wang; Chely M. Garfio; Abigail Vandewalle; Robert C. Spitale

doi:10.1039/D3CC00043E

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Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA†

Mrityunjay Gupta,

^a Jingtian Wang,^b Chely M. Garfio,^b Abigail Vandewalle^c and Robert C. Spitale

*^abc

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA
E-mail: rspitale@uci.edu

^b Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA

^c Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92697, USA

Abstract

We report the detection of 5-vinyluridine (5-VUrd) in RNA at single nucleotide resolution via mutational profiling. Maleimide cycloadducts with 5-VUrd in RNA cause a stop in primer extension during reverse transcription, and the full-length cDNA product from reverse transcription contains misincorporation across the cycloadduct site.

This article is part of the themed collection: 2023 Pioneering Investigators

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Article information

DOI: https://doi.org/10.1039/D3CC00043E
Article type: Communication
Submitted: 03 Jan 2023
Accepted: 07 Feb 2023
First published: 21 Feb 2023

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Chem. Commun., 2023,59, 3257-3260

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Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

M. Gupta, J. Wang, C. M. Garfio, A. Vandewalle and R. C. Spitale, Chem. Commun., 2023, 59, 3257 DOI: 10.1039/D3CC00043E

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Chemical Communications

Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA†

Abstract

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Cycloaddition enabled mutational profiling of 5-vinyluridine in RNA

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