Issue 10, 2023

Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

Abstract

Depicting how biomolecules move and interact within their physiological environment is one of the hottest topics of structural biology. This Feature Article gives an overview of the most recent advances in Site-directed Spin Labeling coupled to Electron Paramagnetic Resonance spectroscopy (SDSL–EPR) to study biomolecules in living cells. The high sensitivity, the virtual absence of background, and the versatility of spin-labeling strategies make this approach one of the most promising techniques for the study of biomolecules in physiologically relevant environments. After presenting the milestones achieved in this field, we present a summary of the future goals and ambitions of this community.

Graphical abstract: Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

Article information

Article type
Feature Article
Submitted
31 Oct 2022
Accepted
04 Jan 2023
First published
05 Jan 2023
This article is Open Access
Creative Commons BY license

Chem. Commun., 2023,59, 1274-1284

Dance with spins: site-directed spin labeling coupled to electron paramagnetic resonance spectroscopy directly inside cells

A. Pierro and M. Drescher, Chem. Commun., 2023, 59, 1274 DOI: 10.1039/D2CC05907J

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