Bioinspired molecular engineering of bivalent aptamers by ligand-induced self-dimerization

Shujuan Xu; Lei He; Fengming Chen; Wenya Dang; Sitao Xie; Long Li; Cheng Cui; Yu Yang; Yanlan Liu; Weihong Tan

doi:10.1039/D2CC05799A

Bioinspired molecular engineering of bivalent aptamers by ligand-induced self-dimerization†

Shujuan Xu,‡^ab Lei He,‡^c Fengming Chen,^a Wenya Dang,^a Sitao Xie,^c Long Li,^e Cheng Cui,*^ae Yu Yang,

^de Yanlan Liu*^a and Weihong Tan

*^acd

Author affiliations

* Corresponding authors

^a Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Aptamer Engineering Center of Hunan Province, Hunan University, Changsha, Hunan 410082, China
E-mail: tan@hun.edu.cn, ylliu@hnu.edu.cn, ccui@hnu.edu.cn

^b Key Lab of Biotechnology and Bioresources Utilization of Ministry of Education, College of Life Science, Dalian Minzu University, Dalian, Liaoning 116600, China

^c Zhejiang Cancer Hospital, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China

^d Institute of Molecular Medicine (IMM), Renji Hospital, Shanghai Jiao Tong University School of Medicine, and College of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China

^e Department of Chemistry and Department of Physiology and Functional Genomics, Center for Research at the Bio/NanoInterface, Health Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, USA

Abstract

A bioinspired “point-and-shoot” molecular strategy is described for engineering noncovalent aptamers via K⁺-induced reassembly of a split G-quadruplex sequence at the end of monomeric aptamers. This design compensates for insufficient cell recognition of monovalent aptamers in biological environments and minimizes structure-induced nonspecific cell binding, expending the aptamer toolbox available for complex systems.

Chemical Communications

Bioinspired molecular engineering of bivalent aptamers by ligand-induced self-dimerization†

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Bioinspired molecular engineering of bivalent aptamers by ligand-induced self-dimerization

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