Issue 11, 2023

A strategy for orthogonal deubiquitination using a bump-and-hole approach

Abstract

We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in living cells.

Graphical abstract: A strategy for orthogonal deubiquitination using a bump-and-hole approach

Supplementary files

Article information

Article type
Communication
Submitted
19 Jun 2023
Accepted
11 Sep 2023
First published
02 Oct 2023
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2023,4, 879-883

A strategy for orthogonal deubiquitination using a bump-and-hole approach

T. Suzuki, Y. Utsugi, S. Yamanaka, H. Takahashi, Y. Sato, T. Sawasaki and Y. Miyamae, RSC Chem. Biol., 2023, 4, 879 DOI: 10.1039/D3CB00095H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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