Issue 15, 2023

Construction of a spheroid array culture system on a suspended permeable hydrogel membrane scaffold for improving the expression of a liver-specific drug-metabolizing enzyme of HepG2 cells

Abstract

Herein, we constructed a spheroid array culture system on a flexible hydrogel membrane suspended in the culture medium. When we applied this culture system to HepG2 cells, the results suggested that an aerobic culture environment was implemented, and the gene expression of a liver-specific drug-metabolizing enzyme was improved in comparison with that of the conventional immobilized monolayer culture.

Graphical abstract: Construction of a spheroid array culture system on a suspended permeable hydrogel membrane scaffold for improving the expression of a liver-specific drug-metabolizing enzyme of HepG2 cells

Supplementary files

Article information

Article type
Communication
Submitted
15 Jan 2023
Accepted
03 Jun 2023
First published
22 Jun 2023

Biomater. Sci., 2023,11, 5129-5135

Construction of a spheroid array culture system on a suspended permeable hydrogel membrane scaffold for improving the expression of a liver-specific drug-metabolizing enzyme of HepG2 cells

A. Tsuyukubo, K. Morishita, T. Kanamori and K. Sumaru, Biomater. Sci., 2023, 11, 5129 DOI: 10.1039/D3BM00071K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements