A dual channel fluorescence tongue for catechin recognition based on the MnO2 nanorods–Amplex Red–o-phenylenediamine reaction system

Abstract

Here, we report a dual-channel fluorescence sensor array for catechin discrimination based on the MnO₂ nanorods (NRs)–Amplex Red (AR)–o-phenylenediamine (OPD) catalytic reaction system. MnO₂ catalyzes both OPD and AR oxidation, and the fluorescence intensity values generated at 550 nm and 590 nm provide “fingerprints” for the sensor array. Different catechins have varying degrees of inhibitory effects on the MnO₂ NRs–AR–OPD catalytic reaction system, thus obtaining unique fluorescence response fingerprints. Through linear discriminant analysis (LDA), the sensor array can not only successfully distinguish 5 catechins with concentrations as low as 500 nM and different concentrations of catechins, but also realize the identification of catechin mixtures. Notably, this method only requires the preparation of a single nanomaterial that catalyzes two substrates simultaneously and can generate two different fluorescence signal outputs, greatly facilitating the design of the sensor array.