Issue 36, 2023

Naked-eye visualization of nucleic acid amplicons using hierarchical nanoassembly

Abstract

This study reports the development of a rapid visualization method for DNA amplicons. Oligonucleotide-coated gold nanoparticles hierarchically assemble on DNA networks to form globular nanostructures, which precipitate into a distinct visible red pellet. This aims to overcome challenges associated with nanoparticle aggregation and dye-based colorimetric detection in LAMP assays.

Graphical abstract: Naked-eye visualization of nucleic acid amplicons using hierarchical nanoassembly

Supplementary files

Article information

Article type
Communication
Submitted
23 Jun 2023
Accepted
03 Aug 2023
First published
03 Aug 2023

Anal. Methods, 2023,15, 4640-4644

Naked-eye visualization of nucleic acid amplicons using hierarchical nanoassembly

V. T. Novi and A. Abbas, Anal. Methods, 2023, 15, 4640 DOI: 10.1039/D3AY01050C

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