Issue 33, 2023

One-step synthesis of a pH switched pyrene-based fluorescent probe for ratiometric detection of HS in real water samples

Abstract

Only a few probes are suited for highly acidic environments and sensitive to pH values below 4. Thus, finding a solution for detecting strong acidic (pH value below 2) conditions is still challenging. Herein, we constructed and created a pH-switched fluorescent probe based on pyrene and a heteroatom containing pyridine unit. When exposed to acidic environments (pH 2.0), the probe's fluorescence redshifted with distinct colour and fluorescence changes owing to protonation on the nitrogen atom containing pyridine moiety, which could be deprotonated by HS selectively compared to other competing analytes. Pyr can detect HS with a rapid response within 5 s and showed very good quantum yield under acidic environments. The sensing mechanism was confirmed by Density Functional Theory (DFT) studies using the B3LYP and 6-31G+ (d) basis sets. Furthermore, the probe was utilized to monitor HS in actual water samples and identify H2S gas by a simple paper strip test.

Graphical abstract: One-step synthesis of a pH switched pyrene-based fluorescent probe for ratiometric detection of HS− in real water samples

Supplementary files

Article information

Article type
Paper
Submitted
15 Jun 2023
Accepted
24 Jul 2023
First published
14 Aug 2023

Anal. Methods, 2023,15, 4159-4167

One-step synthesis of a pH switched pyrene-based fluorescent probe for ratiometric detection of HS in real water samples

K. Magesh, S. Singh, S. P. Wu and S. Velmathi, Anal. Methods, 2023, 15, 4159 DOI: 10.1039/D3AY00987D

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