Issue 25, 2023

Arrayed labeling-free cultivation and growth evaluation from a single microorganism

Abstract

The development of high-throughput screening methods for microorganisms is desired because microorganisms are useful and sustainable resources with which valuable substances utilized in various industries can be produced. Micro-space-based methods are the best candidates for the efficient screening of microorganisms owing to their low reagent consumption and compact integration. In this study, we developed a picoliter-sized incubator array for quantitative and labeling-free evaluation of the growth process of Escherichia coli (E. coli) by autofluorescence. Because the array with 8464 incubators is able to compartmentalize single E. coli individually utilizing the Poisson distribution, the array can evaluate 100 single E. coli simultaneously. Our incubator array not only realized the high-throughput screening of microorganisms, but also provided an analytical tool for assessing individual differences in E. coli.

Graphical abstract: Arrayed labeling-free cultivation and growth evaluation from a single microorganism

Supplementary files

Article information

Article type
Paper
Submitted
16 Mar 2023
Accepted
08 May 2023
First published
08 May 2023

Anal. Methods, 2023,15, 3019-3025

Author version available

Arrayed labeling-free cultivation and growth evaluation from a single microorganism

Y. Tanaka, T. Minamiki and R. Kurita, Anal. Methods, 2023, 15, 3019 DOI: 10.1039/D3AY00401E

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