Issue 23, 2023

A dual mode ‘turn-on’ fluorescence-Raman (SERS) response probe based on a 1H-pyrrol-3(2H)-one scaffold for monitoring H2S levels in biological samples

Abstract

Discovery of the biological signaling roles of H2S has spurred great interest in developing reliable methods for its accurate detection and quantification. As considerable variation in its levels is seen during pathological conditions such as sepsis, real-time quantification methods have relevance in diagnosis as well. Of various approaches, reaction-based probes which respond through ‘off–on’ fluorescence emission remain the most studied. Since the intensity of emission is related to the analyte concentration in these measurements, the presence of built-in features which provide an opportunity for internal referencing will be advantageous. In view of this, a dual mode response system that senses H2S through characteristic fluorescence and Raman (SERS) signals based on a 1H-pyrrol-3(2H)-one scaffold was developed and is the main highlight of this report. This probe offers several advantages such as fast response (<1 min), and high selectivity and sensitivity with a detection limit of ∼7 nM. Imaging of H2S in HepG2 cells, making use of the SERS signal from the thiolysis product is also demonstrated.

Graphical abstract: A dual mode ‘turn-on’ fluorescence-Raman (SERS) response probe based on a 1H-pyrrol-3(2H)-one scaffold for monitoring H2S levels in biological samples

Supplementary files

Article information

Article type
Paper
Submitted
23 Feb 2023
Accepted
29 Apr 2023
First published
05 May 2023

Anal. Methods, 2023,15, 2853-2860

A dual mode ‘turn-on’ fluorescence-Raman (SERS) response probe based on a 1H-pyrrol-3(2H)-one scaffold for monitoring H2S levels in biological samples

A. Panthalattu Parambil, S. Shamjith, J. Kurian, A. Kesavan, A. K. Sen, P. R. Thangaraj, K. K. Maiti and M. K. Manheri, Anal. Methods, 2023, 15, 2853 DOI: 10.1039/D3AY00282A

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