A highly sensitive immunosensor is developed using membrane pores as the recognition interface. In this sensor, a Cu-free click reaction is used to efficiently immobilize antibodies, and the sensor inhibits the adsorption of nonspecific proteins that degrade sensitivity. Furthermore, the sensor demonstrates rapid interleukin-6 detection in the picogram per milliliter range.
H. Okuyama, Y. Kodama, K. Takemura, H. Yamashita, Y. Oshiba and T. Yamaguchi, Anal. Methods, 2023, 15, 1494 DOI: 10.1039/D2AY02110B
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