An electrochemical sensing platform based on carbon black and chitosan-stabilized platinum nanoparticles

Abstract

The versatility of chitosan (Ch) biopolymer as a metallic nanoparticle stabilizing agent and excellent former of thin films on glassy carbon was explored in this work for the sustainable manufacture of novel electrochemical sensors based on carbon black (CB) and chitosan-stabilized platinum nanoparticles (Ch-PtNPs). Platinum nanoparticles highly stabilized by chitosan were easily synthesized at room temperature and characterized by HR-TEM, UV–vis, and voltammetry. Ch-PtNPs presented an average diameter of 2.7 nm, and typical voltammetric peaks of Pt in sulfuric acid medium were detected for films containing Ch-PtNPs. As a proof of concept, the CB-Ch-PtNP electrode was applied in the determination of hydrogen peroxide (H₂O₂) and the endocrine disruptor bisphenol A (BPA). Pronounced electrocatalytic activity towards H₂O₂ reduction was observed in the presence of Ch-PtNPs in the films, guaranteeing the non-enzymatic determination of H₂O₂ by chronoamperometry, with a limit of detection of 10 μmol L⁻¹. In the determination of BPA by differential pulse adsorptive anodic stripping voltammetry (DPAdASV), under optimal experimental conditions, a wide linear response range and a limit of detection at the nanomolar level (7.9 nmol L⁻¹) were achieved. In addition, excellent repeatabilities of sensor response and sensor fabrication, and accuracy in the analysis of natural water samples were obtained.