A dual-emission ratiometric fluorescent sensor based on copper nanoclusters encapsulated in zeolitic imidazolate framework-90 for rapid detection and imaging of adenosine triphosphate

Abstract

Adenosine triphosphate (ATP) is the primary energy carrier for intracellular metabolic processes. Accurate and rapid detection of ATP has important implications for clinical diagnosis. In this work, we reported a dual-emission ratiometric fluorescent probe Cu NCs–Al@ZIF-90 formed by encapsulating copper nanoclusters (Cu NCs) into zeolitic imidazolate framework-90 (ZIF-90) using a simple one-pot method. Cu NCs exhibited a remarkable fluorescence enhancement in the presence of aluminum ions due to the aggregation-induced emission (AIE) properties. When ATP existed, the Zn²⁺ nodes in the MOF material acted as selective sites for ATP recognition, resulting in the cleavage of Cu NCs–Al@ZIF-90. As a consequence, two reverse fluorescence changes were observed from released Cu NCs at 620 nm and imidazole-2-carboxaldehyde (2-ICA) at 450 nm, respectively. With the dual-emission ratiometric strategy, efficient and rapid determination of ATP was realized, giving a detection limit down to 0.034 mM in the concentration range of 0.2 mM to 0.625 mM. The convenient synthesis process and the rapid ATP-responsive ability made the proposed Cu NCs–Al@ZIF-90 probe highly promising in clinical and environmental analysis.