Issue 18, 2023

Glucose sandwich assay based on surface-enhanced Raman spectroscopy

Abstract

A facile and sensitive glucose sandwich assay using surface-enhanced Raman scattering (SERS) has been developed. Glucose was captured by 3-aminopheyonyl boronic acid (APBA) modified Ag nanoparticles decorated onto a polyamide surface. Then, Ag nanoparticles modified with 3-amino-6-ethynylpicolinonitrile (AEPO) and APBA were used as SERS tags. APBA forms specific cis-diol compounds with glucose molecules avoiding interference by other saccharides and biomolecules in urine enabling its selective detection. As the actual Raman reporter, AEPO exhibited a distinctive SERS peak in the Raman silent region, thus increasing the sensitivity of the glucose detection to 10−11 M. Additionally, the developed SERS assay was reusable, and its applicability in artificial urine samples demonstrated future clinical utility confirming the potential of this innovative technology as a diagnostic tool for glucose sensing.

Graphical abstract: Glucose sandwich assay based on surface-enhanced Raman spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
29 Mar 2023
Accepted
13 Jul 2023
First published
17 Jul 2023
This article is Open Access
Creative Commons BY-NC license

Analyst, 2023,148, 4310-4317

Glucose sandwich assay based on surface-enhanced Raman spectroscopy

T. Zhang, R. Lu, G. Wang, X. Sun, J. Li and B. Mizaikoff, Analyst, 2023, 148, 4310 DOI: 10.1039/D3AN00481C

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