Issue 3, 2023

Partial collapse of DNA tetrahedron for miRNA assay with duplex-specific nuclease-assisted amplification

Abstract

We establish a facile electrochemical approach for detecting miRNA. Programmable DNA tetrahedron is designed using thiol groups for electrode modification, the amino group for the localization of electrochemical species and a hairpin structure that responds to target miRNA. In addition, duplex-specific nuclease-assisted amplification helps improve the sensitivity of this biosensor. The target-initiated partial collapse of the DNA tetrahedron event integrates recognition, electrode immobilization, signal recruitment and amplification. By measuring the sharp silver stripping peak, the highly sensitive detection of miRNA is achieved, which also performs satisfactorily challenging biological samples. This method is featured with simple operation, high sensitivity and practical utility, exhibiting great application potential in clinical diagnosis.

Graphical abstract: Partial collapse of DNA tetrahedron for miRNA assay with duplex-specific nuclease-assisted amplification

Supplementary files

Article information

Article type
Communication
Submitted
18 Nov 2022
Accepted
24 Dec 2022
First published
17 Jan 2023

Analyst, 2023,148, 512-515

Partial collapse of DNA tetrahedron for miRNA assay with duplex-specific nuclease-assisted amplification

J. Zhu, Z. Guo, J. Cui and P. Miao, Analyst, 2023, 148, 512 DOI: 10.1039/D2AN01889F

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