Issue 45, 2022

Extremophilic behavior of catalytic amyloids sustained by backbone structuring

Abstract

Enzyme function relies on the placement of chemistry defined by solvent and self-associative hydrogen bonding displayed by the protein backbone. Amyloids, long-range multi-peptide and -protein materials, can mimic enzyme functions while having a high proportion of stable self-associative backbone hydrogen bonds. Though catalytic amyloid structures have exhibited a degree of temperature and solvent stability, defining their full extremophilic properties and the molecular basis for such extreme activity has yet to be realized. Here we demonstrate that, like thermophilic enzymes, catalytic amyloid activity persists across high temperatures with an optimum activity at 81 °C where they are 30-fold more active than at room temperature. Unlike thermophilic enzymes, catalytic amyloids retain both activity and structure well above 100 °C as well as in the presence of co-solvents. Changes in backbone vibrational states are resolved in situ using non-linear 2D infrared spectroscopy (2DIR) to reveal that activity is sustained by reorganized backbone hydrogen bonds in extreme environments, evidenced by an emergent vibrational mode centered at 1612 cm−1. Restructuring also occurs in organic solvents, and facilitates complete retention of hydrolysis activity in co-solvents of lesser polarity. We support these findings with molecular modeling, where the displacement of water by co-solvents leads to shorter, less competitive, bonding lifetimes that further stabilize self-associative backbone interactions. Our work defines amyloid properties that counter classical proteins, where extreme environments induce mechanisms of restructuring to support enzyme-like functions necessary for synthetic applications.

Graphical abstract: Extremophilic behavior of catalytic amyloids sustained by backbone structuring

Supplementary files

Article information

Article type
Paper
Submitted
29 Jul 2022
Accepted
18 Oct 2022
First published
19 Oct 2022

J. Mater. Chem. B, 2022,10, 9400-9412

Author version available

Extremophilic behavior of catalytic amyloids sustained by backbone structuring

M. A. Beasley, A. D. Dunkelberger, M. D. Thum, E. S. Ryland, K. P. Fears, A. B. Grafton, J. C. Owrutsky, J. G. Lundin and C. R. So, J. Mater. Chem. B, 2022, 10, 9400 DOI: 10.1039/D2TB01605B

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