Fluorescence detection and imaging of intracellular sulphite using a remote light activatable photochromic nanoprobe

Abstract

The development of a responsive fluorescent probe for the detection of a particular biomolecule in a specific site at the desired moment is important in the fields of bioanalysis and imaging, molecular biology and biomedical research. In this work, we report the development of a remote-light activatable nanoprobe for the fluorescence detection of sulphite in pure aqueous solution and its imaging applications in living cells. The nanoprobe, Poly-Cm-SP, is fabricated simply by wrapping photochromic molecules (Cm-SP) into a polymer nanoparticle. Upon alternate UV/Vis light irradiation for several seconds, the Poly-Cm-SP nanoprobe exhibits red/blue fluorescence switch due to the inactive/active FRET processes from coumarins to the SP/MR isomers of the photochromic molecule. In the presence of sulphite, the specific reaction of sulphite with the electron deficit “CC” bond of the MR isomer occurs, resulting in an inefficient FRET process and thus exhibiting a constant “ON” blue channel fluorescence signal. After UV-light irradiation, the formation of activated Poly-Cm-MRin situ thus enables the detection of sulphite through recording the ratiometric changes of fluorescence signals at both blue and red channels. The Poly-Cm-SP nanoprobe possesses excellent biocompatibility and lysosome distribution capability, allowing it to be used for photochromic imaging and sulphite detection in the lysosomes of living macrophage cells. This work thus offers a new remote-light activatable nanoprobe for the detection and imaging of sulphite in biological systems.