Cellular micromasonry: biofabrication with single cell precision

S. Tori Ellison; Senthilkumar Duraivel; Vignesh Subramaniam; Fredrik Hugosson; Bo Yu; Joseph J. Lebowitz; Habibeh Khoshbouei; Tanmay P. Lele; Mark Q. Martindale; Thomas E. Angelini

doi:10.1039/D2SM01013E

Cellular micromasonry: biofabrication with single cell precision†

S. Tori Ellison,

^a Senthilkumar Duraivel,^a Vignesh Subramaniam,

^b Fredrik Hugosson,^c Bo Yu,^d Joseph J. Lebowitz,^e Habibeh Khoshbouei,

^e Tanmay P. Lele,

^fgh Mark Q. Martindale^c and Thomas E. Angelini

*^abi

Author affiliations

* Corresponding authors

^a Department of Material Sciences and Engineering, University of Florida, Gainesville, Florida 32611, USA
E-mail: t.e.angelini@ufl.edu

^b Department of Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, USA

^c The Whitney Laboratory for Marine Bioscience, St. Augustine, Florida 32080, USA

^d Department of Chemical Engineering, University of Florida, Gainesville, Florida 32611, USA

^e Department of Neuroscience, University of Florida, Gainesville, Florida 32611, USA

^f Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, USA

^g Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, Texas 77843, USA

^h Department of Translational Medical Sciences, Texas A&M University, Houston, Texas 77843, USA

ⁱ J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, Florida 32611, USA

Abstract

In many tissues, cell type varies over single-cell length-scales, creating detailed heterogeneities fundamental to physiological function. To gain understanding of the relationship between tissue function and detailed structure, and eventually to engineer structurally and physiologically accurate tissues, we need the ability to assemble 3D cellular structures having the level of detail found in living tissue. Here we introduce a method of 3D cell assembly having a level of precision finer than the single-cell scale. With this method we create detailed cellular patterns, demonstrating that cell type can be varied over the single-cell scale and showing function after their assembly.

Soft Matter

Cellular micromasonry: biofabrication with single cell precision†

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