Domain dynamics of phase-separated lipid membranes under shear flow

Abstract

The dynamical behaviour of lateral domains on phase-separated lipid vesicles under external flow is reported. A microfluidic chamber was used for the immobilization of vesicles and the application of shear. Microscopic observation revealed that domains tended to be localized at the vortex center and to exhibit a stripe morphology as the flow speed increased. We clarified the dependency of domain behaviors on the flow speed and lipid mixing fraction. The cholesterol ratio in the membrane affected these domain behaviors. Next, we investigated the growth of domains under flow. We discuss the mechanism of these trends by considering the free energy of phase separation, and reproduce the experimental results by numerical simulations. These findings may lead to a better understanding of the dynamical properties of the membrane under nonequilibrium situations and the biophysical mechanism of cellular mechanotransduction.

Graphical abstract: Domain dynamics of phase-separated lipid membranes under shear flow

Supplementary files

Article information

Article type
Paper
Submitted
21 Jun 2022
Accepted
03 Nov 2022
First published
24 Nov 2022
This article is Open Access
Creative Commons BY-NC license

Soft Matter, 2022, Advance Article

Domain dynamics of phase-separated lipid membranes under shear flow

T. Hamada, S. Mizuno and H. Kitahata, Soft Matter, 2022, Advance Article , DOI: 10.1039/D2SM00825D

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