A green fluorescence turn-off system for meclofenoxate determination by Cilefa Pink B dye

Abstract

Because of their high extinction indices, high quantum yields, and propensity to attach to biomolecules, xanthene dyes, and related compounds have attracted more attention for analytical drug labelling and monitoring. The halogen-substituted xanthene dye, Cilefa Pink B, has also been adapted for usage in the food and pharmaceutical industries as well as for diagnostic purposes. Cilefa Pink B dye is a promising reagent for the quantitative analysis of numerous analytes due to its natural fluorescence characters. MFX is a powerful antioxidant serving as a nootropic agent, motivating glucose uptake and oxygen levels and improving metabolic energy in the brain. The first novel fluorimetric method for quantifying the cerebral circulatory enhancer meclofenoxate is presented in this article. This study used a green, single-pot, and direct fluorimetric strategy to quantify and validate meclofenoxate. A rapid association complex was designed using meclofenoxate and the Cilefa Pink B dye in a weakly acidic solution. The fluorometric assay was performed based on the turn-off effect of meclofenoxate on the fluorescence magnitude of the bio pigment (Cilefa Pink B) at 556.5 nm. The linearity is within the range of 0.08–1.9 μg mL⁻¹. Regarding all system parameters, meclofenoxate–Cilefa Pink B coupled complexes were regulated analytically. The system was compliant with ICH guidelines as well. Meclofenoxate in indicated therapy dosage forms was successfully recovered using the designed procedure. The planned fluorescence detection approach has also been effectively utilized to monitor the analyte of interest in its crude and commercial forms. Additionally, the reaction kinetics were studied further, and the product was characterized and confirmed spectroscopically. An eco-scale was applied to rate the environmental friendliness of the designed method.