Issue 52, 2022, Issue in Progress

New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

Abstract

A new, cost-effective and sensitive spectroscopic assay for the quantification of Colistin Sulfate (CS) and its prodrug colistimethate sodium (CMS) has been developed and validated. The validated technique depends on the condensation of the studied drug with 2,2-dihydroxyindan-1,3-dione (ninhydrin) and phenylacetaldehyde using Teorell and Stenhagen buffer (pH = 6) to yield a fluorescent product that is estimated at emission wavelength (λem = 474 nm) after excitation wavelength (λex = 390 nm). The reaction's affecting factors were carefully studied and adjusted accurately. Over the following range (0.4–2.4 μg mL−1), the produced calibration plot looked rectilinear, and the estimated limits of detection and quantification (LOD and LOQ) were 0.051 & 0.154 μg mL−1 respectively. The recommended approach was utilized to evaluate market products containing the investigated drug. Moreover, content uniformity testing was employed as a new procedure not found in the previously reported fluorimetric technique.

Graphical abstract: New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

Article information

Article type
Paper
Submitted
10 Jul 2022
Accepted
15 Nov 2022
First published
23 Nov 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 33559-33566

New validated spectrofluorimetric protocol for colistin assay through condensation with 2,2-dihydroxyindan-1,3-dione: application to content uniformity testing

T. Z. Attia, M. A. Abdelmajed, M. A. Omar, S. S. Al Thagfan and K. M. Badr El-Din, RSC Adv., 2022, 12, 33559 DOI: 10.1039/D2RA04259B

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