Issue 17, 2022, Issue in Progress

Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

Abstract

We report a universal and signal-on HCR based detection platform via innovatively coupling the CRISPR-Cas12a system with HCR. By using this CRISPR-HCR pathway, we can detect different targets by only changing the crRNA. The CRISPR-HCR platform coupling with an upstream amplifier can achieve a practical sensitivity as low as ∼aM of ASFV gene in serum.

Graphical abstract: Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

Supplementary files

Article information

Article type
Paper
Submitted
28 Feb 2022
Accepted
24 Mar 2022
First published
04 Apr 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 10374-10378

Coupling nucleic acid circuitry with the CRISPR-Cas12a system for universal and signal-on detection

R. Zhao, C. Yu, B. Lu and B. Li, RSC Adv., 2022, 12, 10374 DOI: 10.1039/D2RA01332K

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